AKAP4 is playing a central role in flagellar structure, chemotaxis, capacitation and sperm motility (Luconi et al., 2011).
In mammals, AKAP4 is expressed during spermatogenesis. This 854 amino-acid protein is processed from a pro-AKAP4 to a “mature” AKAP4 protein lacking the 188 first amino acids (Turner et al., 1998).
The AKAP4 precursor termed proAKAP4 is processed to mature AKAP4 during sperm maturation in the human testes (Nipper et al., 2006).
AKAP4 is specifically localized to the fibrous sheath of the principal piece of the flagellum a structure involved in flagellum structure and sperm motility.
Examples of AKAP4 expressions in bull, horse and pig semen
Bull semen Horse semen Pig semen
The mouse monoclonal antibody clone 7E10 (Ref. 4BDX-1602) is a pertinent biomarker to better understand the mechanism of sperm motility in humans and in mammals. This monoclonal antibody will help you to study sperm quality and to better understand male fertility dysfunctions in mammals.
The mouse monoclonal antibody clone 7E10 (Ref. 4BDX-1602) recognizes the Carboxy-terminal region of A-kinase anchor protein 4 (AKAP4) and also teh C-Terminus of the pro-AKAP4 (precursor) and is suitable for Western Blot, Immunohistochemistry, Immunofluorescence and electron microscopy.
The 4BioDx antibody clone 6F12 (Ref. 4BDX-1701) recognizes the precursor of A-kinase anchor protein 4 (AKAP4) called pro-AKAP4. Western blotting and immunocytochemistry techniques using monoclonal antibodies (clone 6F12) showed that the pro-AKAP4 is specifically expressed in human, equine, porcine, bovine murine and ovine spermatozoa.
Our ELISA Kits are complete immunoassays with all reagents and buffers required to quantify proAKAP4 in animal semen samples as a biomarker of sperm quality: