In mammals, proAKAP4 and AKAP4 are both specifically localized to the fibrous sheath of the principal piece of the flagellum and are largely known to be involved in the flagellum structure and sperm motility (Sergeant et al., 2019)

In mammals, proAKAP4 is synthetized from spermatid stage during spermatogenesis and full stock of proAKAP4 is present in the ejaculated spermatozoa. 

The pro-AKAP4 precursor is processed to a “mature” AKAP4 protein that  is playing a central role in flagellar structure, hypermotility, capacitation and fertility.

The amounts of proAKAP4 positive spermaozoa is then predicting sperm quality and male fertility as  found in humans, large animals and small mammals (Delehedde et al., 2019).

Examples of AKAP4 and proAKAP4 expressions in bull and horse  semen

                Bull semen                                             Horse semen                  

The 4MID®  kits are complete ELISA kits with all reagents and buffers to quantify proAKAP4 in semen samples (fresh, chilled or frozen semen) :

• Pig 4MID®  Kit (Pig proAKAP4): Ref. 4VDX-18K2
• Horse 4MID®  Kit (Horse proAKAP4): Ref. 4VDX-18K3
• Bull 4MID®  Kit (Bull proAKAP4): Ref. 4VDX-18K4
• Goat 4MID® Kit (Goat proAKAP4) : Ref. 4VDX-18K9
• Dog 4MID® Kit (Dog proAKAP4) : Ref. 4VDX-18K5
• Ram 4MID® Kit (Ram proAKAP4) : Ref. 4VDX-18K7
• Mouse 4MID® Kit (Mouse proAKAP4) : Ref. 4VDX-18K8
• Rabbit 4MID® Kit (Rabbit proAKAP4) : Ref. 4VDX-18K
• Cat 4MID® Kit (Feline proAKAP4) : Ref. 4VDX-18K10
• Camel 4MID® Kit (Camelid proAKAP4) : Ref. 4VDX-18K11

Examples of applications of the 4MID Kits

Use for select semen before cryopreservation / predict post-thaw motility

Use to predict shelf life

Use to select extenders to improve shelf life, etc.

Please visit also our website www.4biodx-breeding.com dedicated to breeding and insemination centers