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Horse 4MID® kit (proAKAP4 biomarker)

Reference: : 4VDX-18K3

The Horse 4MID® kit (Ref. 4VDX-18K3) allows you to quantify proAKAP4 in samples of horse semen as a biomarker of sperm quality.

100 Items

969,00 €

Data sheet


Horse proAKAP4

Analytical range

2,34 ng to 150 ng

Sample type

Horse semen


User instructions ref. 4VDX-18K3

More info

Innovative immunoassay to assess semen quality in horse

The Horse 4MID® kit (Ref. 4VDX-18K3) is the first immunoassay that validates objectively semen quality by quantifying a protein biomarker named proAKAP4 in samples of horse semen. This ELISA kit is the 1st functional test to evaluate sperm quality.

The dosage of the proAKAP4 biomarker with the Horse 4MID® kit will bring you:

Tool for the selection of the breeding males
• Qualification of each breeding male
• Qualification of male ejaculates before and after cryopreservation

The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecondation.

Studies are currently conducted together with the "European Horse Center of Mont Le Soie Linalux" (Belgium) and the research teams of SPQI - 4BioDx.

Technical data

  • Name: Horse 4MID kit
  • Up to 80 samples can be run by kit
  • Working with fresh ejaculate or with frozen semen in extenders
  • Easy to use immunoassay / Manual and automated assay
  • Robust and reproductible method

Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.

Reagents provided in the Horse 4MID®

he Horse 4MID® kit (Ref. 4VDX-18K3) contains all reagents and buffers required for the quantification of proAKAP4 protein in horse semen.

  • One Microplate pre-coated with the Capture Antibody
  • One vial of Standard
  • One vial of Detection Antibody
  • One bottle of the Enzyme Reagent
  • One bottle of 10X Wash Buffer
  • One bottle of the Sample Diluant
  • One bottle of the Acid Stop Solution
  • One Vial of Positive Control
  • Two ELISA plate sealers

Please visit our website to discover our tools dedicated to breeding and insemination centers.