proAKAP4 ELISA Kit - Rodent and human proAKAP4

A robust testing kit to assess semen quality and male fertility in all rodents and primates (including human).    

The proAKAP4 ELISA Kit - Rodent and human proAKAP4 contains all reagents and buffers required for the quantification of proAKAP4 protein in rodent and human sperm samples (research and preclinical studies). 

This ELISA Kit is the 1st testing kit to evaluate sperm quality and spermatozoa functionality in vitro.

The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient over time (Sergeant et al. 2019).

High concentrations of #proAKAP4 help select semen with long lasting motility and are indicative of good spermatogenesis, giving more mature, more “normal’ spermatozoa and less tail abnormalities ratio in semen samples.

Technical data

• Name: proAKAP4 ELISA Kit - Format BB
• Specificity : Rodent and human proAKAP4
• This ELISA kit allow to assay from 1 to 24samples simultaneously or individually (12 strips of 8 wells per plate / kit)
• Working with fresh, chilled or frozen spermatozoa / isolated spermatozoa
• Easy to use immunoassay 
• Easy to interpret 
• Robust, objective and reproductible method

Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.

References about AKAP4/proAKAP4 :

• Carracedo S, Briand-Amirat L, Dordas-Perpinyà M, Ramos Escuredo Y, Delcombel R, Sergeant N and Delehedde M (2022) ProAKAP4 protein marker: towards a functional approach to male fertility. Animal Reproduction Science. Vol.247(107074):1-20. https://doi.org/10.1016/j.anireprosci.2022.107074
• Delehedde M, Carracedo S, Selleslagh M, et al. ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. (2019);2(1):13-19
• Bastan I and Akcay E (2021) Quality assessment of frozen bull semen with the precursor A-kinase anchor protein 4 biomarker. Andrologia e14164. 
• Delehedde M, Demouveaux B, Remy G, Selleslagh M, Dewulf Q, Desseyn JL, Moreau M, Gosset P, Muriel Pichavant M and Sergeant N (2019a) ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. Vol. 7(1):86.
• Riesco M, Anel-Lopez L, Neila-Montero M, Palacin-Martinez C, Montes-Garrido R, Alvarez M, de Paz P, Anel L (2020) ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm. Biomolecules. 10(7):1046. doi: 10.3390/biom10071046
• Nixon B, Bernstein I, Cafe SL, Delehedde M, Sergeant S, Eamens AL, Lord T, Dun MD, De Iuliis GN and Bromfield EG (2019b) A Kinase Anchor Protein 4 is vulnerable to 
• Sergeant N, Briand-Amirat L, Bencharif D and Delehedde M (2019) The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. Vol. 11:01-19.
• Brown PR, Miki K, Harper D and Eddy EM (2003) A-kinase anchoring protein 4 binding proteins in the fibrous sheath. Biology of Reproduction. Vol. 68:2241-2248.
• Miki K, Goulding EH, Fulcher KD, Eddy E, Willis WD and Brown PR (2002) Targeted disruption of the AKAP4 gene causes defects in sperm flagellum and motility. Developmental Biology. Vol. 248:331-342.