Horse 4MID® Kit - Format BB (2 tests)

Innovative assay to quantify proAKAP4 in stallion semen as a molecular and objective semen quality assessment. 

The Horse 4MID® Kit (Ref. 4VDX-18K3BB) is the format that that allows to quantify 1 or 2 analysis per strip then from 12 to 24 analysis per kit. As his homolog, this innovative testing kit allows to quantify proAKAP4 in horse semen as a biomarker of sperm quality and stallion fertility.

This first testing kit validates objectively semen quality by quantifying a protein named proAKAP4 in samples of horse semen. 

This ELISA kit is the 1st functional testing kit to evaluate sperm quality and male fertility in horse and also donkey.

The dosage of the proAKAP4 marker with the Horse 4MID® Kit - format BB brings you: 

• Qualification of male ejaculate before and after cryopreservation
• Qualification of each breeding male
• Best semen for cryopreservation
• Selection of semen with long lasting motility and functionality at post-thaw
• Selection of semen with more mature, normal and less-tail abnormalities (indicator of good quality spermatogenesis
• Selection of best extender for stallion semen preservation

The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient overtimes (Sergeant et al., 2019).

High concentrations of proAKAP4 help select semen with long lasting motility and are indicative of good spermatogenesis, giving more mature, more “normal’ spermatozoa and less tail abnormalities ratio in bull semen samples (Bastan and Akcay, 2021).

Technical data

• Name: Horse 4MID® Kit
• Reference : 4VDX-18K3BB
• This format kit allows to test from 1 to 2 analysis at the time (12 breakable strips of 8 wells per 96-well microplate / per kit) 
• Working with fresh, chilled or frozen semen in extenders / on isolated spermatozoa
• Easy to use immunoassay 
• Easy to interprete / to implement in breeding centers
• Robust, objective and reproductible method

This format BB of the Horse 4MID Kit (ref. 4VDX-18K3BB) allows from 12 to 24 analysis per kit.

Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.

Main references about AKAP4/proAKAP4 marker :

• Carracedo S, Briand-Amirat L, Dordas-Perpinyà M, Ramos Escuredo Y, Delcombel R, Sergeant N and Delehedde M (2022) ProAKAP4 protein marker: towards a functional approach to male fertility. Animal Reproduction Science. Vol.247(107074):1-20. https://doi.org/10.1016/j.anireprosci.2022.107074
• Smith MZY, Dilger A, Bryan E, Bytnar G, Choi S, Cook K and Kline K (2022) Preliminary Study of Effects of Centrifugation of Stallion Semen on Motility and Pro-AKAP4 Concentration over 72 Hours Post-collection. Journal of Agricultural Science and Technology. Vol (12): 65-72.
• Dordas-Perpinyà M, Sergeant N, Ruelle I, Bruyas JF, Charreaux C, Michaud S, Carracedo S, Catalán J, Miró J, Delehedde M and Briand-Amirat L (2022) ProAKAP4 semen concentrations as a valuable marker protein of post-thawed semen quality and bull fertility: a retrospective study. Veterinary Sciences. Vol. 9 :224. https://doi.org/10.3390/vetsci9050224
• Dordas-Perpinya M, Yanez I, Catalan J, Eddarkaoui S, Delehedde M, Sergeant N, Bruyas JF, Briand-Amirat L, and Miró J (2022) ProAKAP4 concentrations in stallion and donkey: Comparison with kinetic parameters, motility and concentration. Reproduction in Domestic Animals. Vol. 57(1):61.
• Dordas-Perpinyà M, Yanez I, Catalan J, Delehedde M, Sergeant N, Bruyas JF, Briand-Amirat L and Miró J (2022) ProAKAP4 correlation with motion parameters and spermatozoa subpopulations in stallion. Reproduction in Domestic Animals. In Press.
• Dordas-Perpinyà M, Sergeant N, Yanez-Ortiz Y, Catalan J, Delehedde M, Bruyas JF, Briand-Amirat L and Miró J (2022) Characterization of proAKAP4 expression, metabolism and localization in Equus asinus spermatozoa. Reproduction, Fertility and Development. In press.
• Blommaert D, Sergeant N, Delehedde M, Donnay I, Lejeune JP, Franck T and Serteyn D (2021) First results about ProAKAP4 concentration in stallion semen after cryopreservation in two different freezing media. Cryobiology. Vol. 102:133-135.
• Bastan I and Akcay E (2021) Quality assessment of frozen bull semen with the precursor A-kinase anchor pro-tein 4 biomarker. Andrologia e14164.
• Blommaert D, Franck T, Lejeune JF, Selleslagh M, Jouy N, Sergeant N, Serteyn D and Delehedde M (2019) The Horse 4MID® Kit as a promising tool to compare different freezing media to preserve stallion semen. Rev. Bras. Reprod. Anim. Vol 43(2):585.
• Delehedde M, Carracedo S, Selleslagh M, et al. ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. (2019);2(1):13-19
• Sergeant N, Briand-Amirat L, Bencharif D and Delehedde M (2019) The Sperm Specific Protein ProAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. Vol. 11:01-19.
• Nixon B, Bernstein I, Cafe SL, Delehedde M, Sergeant S, Eamens AL, Lord T, Dun MD, De Iuliis GN and Bromfield EG (2019b) A Kinase Anchor Protein 4 is vulnerable to oxidative adduction in male germ cells. Frontiers in Cell and Developmental Biology – Vol. 7:319.
• Blommaert D, Sergeant N, Delehedde M, Jouy N, Mitchell V, Franck T, Donnay I, Lejeune JP and Serteyn D (2019) Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: Promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology. Vol. 131:52-60.
• Delehedde M, Blommaert D, Jouy N, Scabello J, Miersman H, Franck T, Serteyn D, Mitchell V and Sergeant S (2018) Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. Animal Reproduction Science. Vol. 194:24.
• Blommaert D, Sergeant N, Delehedde M, Franck T, Lejeune JP and Serteyn D (2018) Significant correlation between the proAKAP4 concentration and the total and progressive motility in stallion sperm after thawing. Journal of Equine Veterinary Science. Vol. 66:43.
• Sergeant N, Jumeau F, Eddarkaoui S, Sigala J, Dossou GF, Delehedde M, Buee L, Yvoz JF and Mitchell V (2016) Investigating proteomic methods and tools to assess sperm quality. Animal Reproduction Science. Vol. 169:99-135.
• Turner R, Casas-Dolz P, Schlingmann K and Hameed S (2005) Characterization of an A-kinase anchor protein in equine spermatozoa and examination of the effect of semen cooling and cryopreservation on the binding of that protein to the regulatory subunit of protein kinase-A. American Journal of Veterinary Research. Vol. 66:1055-1056.

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