Mouse 4MID® Kit (proAKAP4 marker)

Innovative and functional assay to quantify proAKAP4 in mouse and rat sperm (all rodents).    

The Mouse 4MID® Kit (Ref. 4BDX-18K8) contains all reagents and buffers required for the quantification of proAKAP4 protein in the sperm of main rodents. 

This ELISA Kit is the 1st functional test to evaluate sperm quality in rodents.

The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient overtimes (Sergeant et al. 2019 ; Delehedde et al. 2029).

High concentrations of proAKAP4 help select semen with long lasting motility and are indicative of good spermatogenesis, giving more mature, more “normal’ spermatozoa and less tail abnormalities ratio in semen samples (Sergeant et al. 2019 ; Bastan and Akcay, 2021).

Technical data about the Mouse 4MID® Kit :

• Name: Mouse 4MID® Kit
• Reference: 4BDX-18K8
• Specificity : Mouse proAKAP4  (all rodents)
• This ELISA kit allows possible to test from 1 to 88 samples simultaneously or individually (12 strips of 8 wells per microplate of 96 wells / per kit)
• Easy to use immunoassay
• Easy to implement in main labs / cost-effective method
• Objective, robust and reproductible method

Do not hesitate to contact us if you run only one or two samples at the time. We have a special format called the Mouse 4MID Kit - BB (ref. 4VDX-18K8BB). Avalaible on request at contact@4biodx.com   

Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.

REFERENCES

• Oh EY, Chandanee MR, Dissanayake WMN, Lee SM and Yi YJ (2023) Comparison of fertility competence in toll-like receptor 4 (TLR4)-knock out male mice fed a high-fat diet. J Biomed Transl Res Vol. 24(2):41-52. https://doi.org/10.12729/jbtr.2023.24.2.41
• Boersma A, Primus J, Wagner B, Broukal V, Andersen L, Pachner B, Dahlhoff M, Rülicke T and Auer KE (2022) Influence of sperm cryopreservation on sperm motility and proAKAP4 concentration in mice. Reprod Med Biol. Vol. 21:e12480. doi: 10.1002/rmb2.12480

• Carracedo S, Briand-Amirat L, Dordas-Perpinyà M, Ramos Escuredo Y, Delcombel R, Sergeant N and Delehedde M (2022) ProAKAP4 protein marker: towards a functional approach to male fertility. Animal Reproduction Science. Vol.247(107074):1-20. https://doi.org/10.1016/j.anireprosci.2022.107074
• Carracedo S, Loyens A, Eddarkaoui S, Serteyn D, Malo C, Skidmore L, Briand-Amirat L, Barbotin AL, Maurage CA, Delehedde M and Sergeant N (2020) The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals. Animal Reproduction Science. Vol. 220 :106448
• Delehedde M, Carracedo S, Duchene B, Demouveaux B, Remy G, Kervoaze G, Aubry S, Gevaert MH, Maurage CA, Jonckheere N, Van Seuningen I, Philippe Gosset G, Desseyn JL, Sergeant N and Pichavant M (2020) Cigarette smoking affects the sperm-specific proAKAP4 concentrations and impairs both spermatogenesis and sperm quality. Andrology. Vol. 8 (Suppl. 1):45-46.
• Nixon B, Bernstein I, Cafe SL, Delehedde M, Sergeant S, Eamens AL, Lord T, Dun MD, De Iuliis GN and Bromfield EG (2019b) A Kinase Anchor Protein 4 is vulnerable to oxidative adduction in male germ cells. Frontiers in Cell and Developmental Biology – Vol. 7:319.
• Delehedde M, Demouveaux B, Remy G, Selleslagh M, Dewulf Q, Desseyn JL, Moreau M, Gosset P, Muriel Pichavant M and Sergeant N (2019a) ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. Vol. 7(1):86. 
• Delehedde M, Carracedo S, Selleslagh M, et al. ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. (2019);2(1):13-19.
• Fang X, Huang LL, Xu J, Ma CQ, Chen ZH, Zhang Z, Liao CH, Zheng SX, Huang P, Xu WM, Li N, Sun L (2019) Proteomics and single-cell RNA analysis of Akap4-knockout mice model confirm indispensable role of Akap4 in spermatogenesis. Developmental Biology. 454(2):118-127.
• Delehedde M, Demouveaux B, Remy G, Selleslagh M, Dewulf Q, Desseyn JL, Moreau M, Gosset P, Muriel Pichavant M and Sergeant N (2019a) ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. Vol. 7(1):86.
• Sergeant N, Briand-Amirat L, Bencharif D and Delehedde M (2019) The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. Vol. 11:01-19..
• Miki K, Goulding EH, Fulcher KD, Eddy E, Willis WD and Brown PR (2002) Targeted disruption of the AKAP4 gene causes defects in sperm flagellum and motility. Developmental Biology. Vol. 248:331-342.