Camel 4MID® Kit (proAKAP4 marker)

Innovative complete immunoassay to assess semen quality in camelid sperm.     

The Camel 4MID® Kit (Ref. 4VDX-19K11) contains all reagents and buffers required for the quantification of proAKAP4 protein in sperm of camelids (all breeds).

This ELISA Kit is the 1st functional test to evaluate sperm quality in camelids.

The concentration of the proAKAP4 protein is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecundation (Sergeant et al., 2019 ; Malo et al. 2021)

High concentrations of proAKAP4 help select semen with long lasting motility and are indicative of good spermatogenesis, giving more mature, more “normal’ spermatozoa and less tail abnormalities ratio in semen samples (Sergeant et al. 2019 ; Malo et al. 2031 ; Bastan et Askay, 2021).

Technical data :

• Name: Camel 4MID® Kit
• Reference: 4VDX-19K11
• Specificity: proAKAP4 from camelids (camel, llama, alpaca)
• Ref.: 4BDX-19K11
• This ELISA kit allows to assess from 1 to 88 samples simultaneously or individually (12 breakable strips of 8 wells per 96 wells microplate / per kit).
• Easy to use immunoassay 
• Easy to interpret (give a number)
• Robust, objective and reproductible method

Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.

Main references about AKAP4/proAKAP4:

• Malo C, Carracedo S, Delehedde M, Sergeant N and Skidmore L (2021) Identification of proAKAP4 in Dromedary sperm and their correlation with monthly semen parameters. Reproduction & Fertility. Vol.2(4):268-279.

• Carracedo S, Briand-Amirat L, Dordas-Perpinyà M, Ramos Escuredo Y, Delcombel R, Sergeant N and Delehedde M (2022) ProAKAP4 protein marker: towards a functional approach to male fertility. Animal Reproduction Science. Vol.247(107074):1-20. https://doi.org/10.1016/j.anireprosci.2022.107074

• Riesco MF, Neila-Montero M, Palacín-Martínez C, Montes-Garrido R, Álvarez R; Boixo JC; De Paz P and Anel L and Anel-López L (2022) Establishment of innovative biomarkers to optimize cooling and cryopreservation protocols in ram sperm. Reproduction in Domestic Animals. Vol. 57(1):101.

• Dordas-Perpinya M, Yanez I, Catalan J, Eddarkaoui S, Delehedde M, Sergeant N, Bruyas JF, Briand-Amirat L, and Miró J (2022) ProAKAP4 concentrations in stallion and donkey: Comparison with kinetic parameters, motility and concentration. Reproduction in Domestic Animals. Vol. 57(1):61.

• Kowalczyk A, Gałeska E and Bubel A (2022) The concentration of proAKAP4 and other indicators of cryopotential of spermatozoa cryopreserved in extender with holothuroidea extract addition. Animals. Vol. 12:521.

• Bastan I and Akcay E (2021) Quality assessment of frozen bull semen with the precursor A-kinase anchor pro-tein 4 biomarker. Andrologia e14164.
• Riesco M, Anel-Lopez L, Neila-Montero M, Palacin-Martinez C, Montes-Garrido R, Alvarez M, de Paz P, Anel L (2020) ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm. Biomolecules. 10(7):1046. doi: 10.3390/biom10071046.
• Sergeant N, Briand-Amirat L, Bencharif D and Delehedde M (2019) The Sperm Specific Protein ProAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. Vol. 11:01-19.
• Delehedde M, Carracedo S, Selleslagh M, et al. ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. (2019);2(1):13-19.
• Nixon B, Bernstein I, Cafe SL, Delehedde M, Sergeant S, Eamens AL, Lord T, Dun MD, De Iuliis GN and Bromfield EG (2019b) A Kinase Anchor Protein 4 is vulnerable to oxidative adduction in male germ cells. Frontiers in Cell and Developmental Biology – Vol. 7:319.
• Blommaert D, Sergeant N, Delehedde M, Jouy N, Mitchell V, Franck T, Donnay I, Lejeune JP and Serteyn D (2019b) Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: Promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology. Vol. 131:52-60.
• Delehedde M, Blommaert D, Jouy N, Scabello J, Miersman H, Franck T, Serteyn D, Mitchell V and Sergeant S (2018) Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. Animal Reproduction Science. Vol. 194:24.
• Sergeant N, Jumeau F, Eddarkaoui S, Sigala J, Dossou GF, Delehedde M, Buee L, Yvoz JF and Mitchell V (2016) Investigating proteomic methods and tools to assess sperm quality. Animal Reproduction Science. Vol. 169:99-135.
• Luconi M, Cantini G, Baldi E and Forti G (2011) Role of A-kinase anchoring proteins (AKAPs) in reproduction. Frontiers in Bioscience. Vol. 16:1315-1330.
• Miki K, Goulding EH, Fulcher KD, Eddy E, Willis WD and Brown PR (2002) Targeted disruption of the AKAP4 gene causes defects in sperm flagellum and motility. Developmental Biology. Vol. 248:331-342.

Complete list of references on proAKAP4/AKAP4 sperm quality marker upon request at contact@4biodx.com

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