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Ram 4MID® Kit (proAKAP4 marker)

Reference: : 4VDX-18K7

The Ram 4MID® Kit (Ref.4VDX-18K7) allows you to quantify proAKAP4 in samples of ram semen as a biomarker of sperm quality.

Data sheet




Ram proAKAP4 (all breeds)

Analytical range

0 to 150 ng

Sample type

Fresh, chilled and frozen semen / isolated spermatozoa

More info

Innovative complete immunoassay to assess semen quality in ram  (all breeds). 

The Ram 4MID® Kit (Ref. 4VDX-18K7) is the first immunoassay that validates objectively semen quality by quantifying a protein biomarker named proAKAP4 in samples of ram semen.

This ELISA kit is the 1st functional test to evaluate sperm quality in rams (all breeds).

The dosage of the proAKAP4 biomarker with the Ram 4MID® Kit, will bring:

  • Objective information on semen quality
  • Qualification of each breeding male and their ejaculates
  • Pertinent parameter to improve sperm cryopreservation
  • Selection of semen with long-lasting motility at post-thaw
  • A functional indicator correlated to fertility 

The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient overtimes (Sergeant et al., 2019).

High concentrations of proAKAP4 help select semen with long lasting motility and are indicative of good spermatogenesis, giving more mature, more “normal’ spermatozoa and less tail abnormalities ratio in bull semen samples (Bastan and Akcay, 2021).

proAKAP4 values

Technical data of the Ram 4MID® Kit:

  • Name: Ram 4MID® Kit
  • Specificity : ram proAKAP4 (all breeds)
  • This ELISA kit makes it possible to test from 1 to 88 samples simultaneously or individually (12 strips of 8 wells per microplate of 96 wells / per kit)
  • Working with ejaculate, chilled or frozen semen 
  • Easy to use immunoassay 
  • Easy to interpret 
  • Robust, objective and reproductible method

Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.

References about AKAP4/proAKAP4 marker for semen quality assessments:

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