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Dog 4MID® Kit (proAKAP4 marker)

Reference: : 4VDX-18K5

The Dog 4MID® Kit (Ref. 4VDX-18K5) allows you to quantify proAKAP4 in samples of dog semen as a biomarker of sperm quality and fertility.

Data sheet




dog proAKAP4 (all breeds)

Analytical range

0 to 150 ng

Sample type

Fresh, chilled and frozen spermatozoa / straw

More info

The Dog 4MID® Kit (Ref.4VDX-18K5) is the first testing kit that predicts long lasting semen motility, quality and male fertility. 

The Dog 4MID® Kit validates objectively semen  quality by quantifying a protein biomarker named proAKAP4 in dog semen.

An innovative approach that brings semen analysis to the next level! 

This ELISA kit is the 1st functional test to evaluate sperm quality in dogs (all breeds).

The dosage of the proAKAP4 concentration with the Dog 4MID® Kit allows :

  • Qualification of male ejaculates before and after cryopreservation
  • Qualification of each breeding male
  • Selection of the best semen for cryopreservation
  • Selection of semen with long lasting motility and functionality at post-thaw
  • Selection of semen with more mature, normal and less-tail abnormalities (indicator of good quality spermatogenesis 
  • Selection of best extender for semen preservation

The concentration of the proAKAP4 marker is an objective indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient overtimes (Sergeant et al. 2019 ; Bastan and Akay, 2021).

proAKAP4 values

High concentrations of proAKAP4 help select semen with long lasting motility and are indicative of good spermatogenesis, giving more mature, more “normal’ spermatozoa and less tail abnormalities ratio in bull semen samples (Bastan and Akcay, 2021).

Technical data about the Dog 4MID® Kit :

  • Name: Dog 4MID® Kit
  • Reference : 4VDX-18K5
  • Specificity : dog proAKAP4 (all breeds)
  • ELISA Kit (96 wells microplate)
  • This ELISA kit allow to assess from 1 to 88 samples simultaneously or individually 12 breakable strips of 8 wells per plate/kit)
  • Working with fresh ejaculate, chilled or frozen semen in extenders / isolated spermatozoa
  • Easy to use immunoassay
  • Easy to interpret / to implement in clinics and breeding centers
  • Robust, objective and reproductible method

Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.

Do not hesitate to contact us if you run only one or two samples at the time. We have a special format of this format called the Dog 4MID Kit - BB (ref. 4VDX-18K5BB). Avalaible on request at   

References about AKAP4/proAKAP4 : 

Please visit our website to discover our tools dedicated to breeding and insemination centers.