Dog 4MID® Kit (proAKAP4 biomarker)

The Dog 4MID® Kit (Ref.4VDX-18K5) is the first immunoassay that validates objectively semen quality by quantifying a protein biomarker named proAKAP4 in dog semen. This ELISA kit is the 1st functional test to evaluate sperm quality.

The dosage of the proAKAP4 biomarker with the 4MID® Kit, will bring you:

• Objective information on semen quality
• Qualification of each breeding male and their ejaculates
• Pertinent parameter to improve sperm cryopreservation
• A functional indicator correlated to fertility and prolificity

The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient up to fecondation.

Technical data

• Name: Dog 4MID® Kit
• This kit makes it possible to test 42 samples in duplicate or 87 samples in monoplicate in less than 2 hours
• Working with fresh ejaculate or with frozen semen 
• Easy to use immunoassay / Manual and automated assay
• Robust and reproductible method

Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.

Reagents provided in the Dog 4MID® Kit

The Dog 4MID® Kit (Ref. 4VDX-18K5) contains all reagents and buffers required for the quantification of proAKAP4 protein in dog semen.

• One Microplate pre-coated with the Capture Antibody
• One vial of Standard
• One vial of Detection Antibody
• One bottle of the Enzyme Reagent
• One bottle of 10X Wash Buffer
• One bottle of the Sample Diluant
• One bottle of the Acid Stop Solution
• One Vial of Positive Control
• Two adhesive plate sealers

References about AKAP4/proAKAP4 in dogs: 

• Le Couazer D, Delehedde M, Ruelle I, Sergeant N, Michaud S, Briand L and Bencharif D (2019a) ProAKAP4 as a valuable marker to assess sperm quality in dogs. Reproduction in Domestic Animals. In press.
• Sergeant N, Briand-Amirat L, Bencharif D and Delehedde M (2019) The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. Vol. 11:01-19.
• Le Couazer D, Sergeant N, Jouy N, Loyens A, Destrumelle S, Delehedde M, Briand-Amirat L and Bencharif D (2019b) Expression of proAKAP4 in dog semen as promising marker of sperm quality. Reproduction in Domestic Animals. In press.
• Delehedde M, Blommaert D, Jouy N, Scabello J, Miersman H, Franck T, Serteyn D, Mitchell V, and Sergeant S (2018) Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. Animal Reproduction Science. Vol. 194:24.
• Sergeant N, Jumeau F, Eddarkaoui S, Sigala J, Dossou GF, Delehedde M, Buee L, Yvoz JF and Mitchell V (2016) Investigating proteomic methods and tools to assess sperm quality. Animal Reproduction Science. Vol. 169:99-135.
• Teijeiro JM and Marini PE (2012) The effect of oviductal deleted in malignant brain tumor 1 over porcine sperm is mediated by a signal transduction pathway that involves pro-AKAP4 phosphorylation. Reproduction. Vol. 143:773-785.

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