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Anti-proAKAP4 (clone 6F12) - 100 µg

Reference: : 4BDX-1701

Mouse monoclonal antibody clone 6F12 recognizes the prodomain of A-kinase anchor protein 4 (AKAP4) and is suitable for Western Blot, Immunofluorescence, Flow cytometry and Immunohistochemistry.

329,00 €

Data sheet

Applications

Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Reactivity

Human, Horse, Bull, Mouse, Ram, Rat

Formulation

1 mg/mL in PBS

Conservation

+4°C / -20°C

Quantity

100 µL

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Datasheet 4BDX-1701

More info

The anti-proAKAP4 clone 6F12 recognizes the prodomain of A-kinase anchor protein 4 (AKAP4). This antibody is suitable for Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry.

This antibody was produced from a mouse hybridoma resulting from a mouse immunized with a peptide covering the prodomain of human AKAP4 protein sequence (Uniprot ref. Q5JQC9) which is 70% homologous between mammals. The anti-pro AKAP4 (clone 6F12) antibody epitope is 100% homologous between mammals.

Determined by its ability to recognise the prodomain of human AKAP4 protein. This monoclonal antibody (clone 6F12) only recognizes the pro-form of AKAP4 (110 kDa / 854 AA), the prodomain (21 kDa) and does not react with the AKAP4 (82 kDa / 665 AA). It reacts also with AKAP4 proteins from horse, bull, mouse, rat and ram semens.


Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.



Background on AKAP4 protein

Pro-AKAP4 is processed into mature AKAP4 by proteolytic cleavage of the amino-terminal prodomain (see picture).

AKAP4 (A-Kinase Anchor Protein 4) protein belongs to the family of A-kinase anchor proteins (AKAPs) all sharing a common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the PKA holoenzyme to discrete locations within the cell.


The human AKAP4 gene is located on chromosome X and encodes a 854 amino acid polypeptide localized in the fibrous sheath of the sperm flagellum, AKAP4 plays a major role in sperm motility.

AKAP4 is also named AKAP-4, AKAP82 (A-Kinase Anchor Protein 82 KDa), PRKA4 (Protein Kinase A Anchoring Protein 4), HI, CT99 (Cancer/Testis Antigen 99), FSC1 (Fibrous sheath component 1) or P82.

The monoclonal antibody (clone 6F12) only recognizes the pro-form of AKAP4. It does not react with the cleaved forms AKAP4.

Western blotting and immunocytochemistry techniques using the monoclonal anti-pro AKAP4 antibody clone 6F12 showed that the pro-AKAP4 is specifically expressed in human, equine, porcine, bovine murine and ovine spermatozoa.

Mouse monoclonal antibody clone 6F12 is suitable for Western Blot, Immunofluorescence, ELISA and Immunohistochemistry (see applications).

Applications

Western Blot (Human spermatozoa)

Western Blot (Human spermatozoa)

Immunofluorescence (Human spermatozoa)

Immunofluorescence (Human spermatozoa)

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