Reference: : 4BDX-1701
Mouse monoclonal antibody clone 6F12 recognizes the prodomain of A-kinase anchor protein 4 (AKAP4) and is suitable for Western Blot, Immunofluorescence, Flow cytometry and Immunohistochemistry.
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|Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry|
|Human, Horse, Bull, Mouse, Ram, Rat|
|1 mg/mL in PBS|
|+4°C / -20°C|
| Datasheet 4BDX-1701|
The anti-proAKAP4 clone 6F12 recognizes the prodomain of A-kinase anchor protein 4 (AKAP4). This antibody is suitable for Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry.
This antibody was produced from a mouse hybridoma resulting from a mouse immunized with a peptide covering the prodomain of human AKAP4 protein sequence (Uniprot ref. Q5JQC9) which is 70% homologous between mammals. The anti-pro AKAP4 (clone 6F12) antibody epitope is 100% homologous between mammals.
Determined by its ability to recognise the prodomain of human AKAP4 protein. This monoclonal antibody (clone 6F12) only recognizes the pro-form of AKAP4 (110 kDa / 854 AA), the prodomain (21 kDa) and does not react with the AKAP4 (82 kDa / 665 AA). It reacts also with AKAP4 proteins from horse, bull, mouse, rat and ram semens.
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
Pro-AKAP4 is processed into mature AKAP4 by proteolytic cleavage of the amino-terminal prodomain (see picture).
AKAP4 (A-Kinase Anchor Protein 4) protein belongs to the family of A-kinase anchor proteins (AKAPs) all sharing a common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the PKA holoenzyme to discrete locations within the cell.
The human AKAP4 gene is located on chromosome X and encodes a 854 amino acid polypeptide localized in the fibrous sheath of the sperm flagellum, AKAP4 plays a major role in sperm motility.
AKAP4 is also named AKAP-4, AKAP82 (A-Kinase Anchor Protein 82 KDa), PRKA4 (Protein Kinase A Anchoring Protein 4), HI, CT99 (Cancer/Testis Antigen 99), FSC1 (Fibrous sheath component 1) or P82.
The monoclonal antibody (clone 6F12) only recognizes the pro-form of AKAP4. It does not react with the cleaved forms AKAP4.
Western blotting and immunocytochemistry techniques using the monoclonal anti-pro AKAP4 antibody clone 6F12 showed that the pro-AKAP4 is specifically expressed in human, equine, porcine, bovine murine and ovine spermatozoa.
Mouse monoclonal antibody clone 6F12 is suitable for Western Blot, Immunofluorescence, ELISA and Immunohistochemistry (see applications).