Reference: : 4BDX-1901S
Mouse monoclonal antibody clone 5H2 recognizes the precursor of the A-kinase anchor protein 4 called proAKAP4 and is suitable for Western Blot, Immunofluorescence, Flow cytometry and Immunohistochemistry applications.
Warning: Last items in stock!
|Western Blot , Immunohistochemistry, Immunofluorescence, Electron Microscopy, Flow cytometry|
|Goat, Camel, Cat, Dog, Pig, Rabbit,|
|1 mg/mL in PBS|
|+4°C / -20°C|
| Fiche technique 4BDX-1901S|
The anti-proAKAP4 clone 5H2 recognizes the precursor of AKAP4 that is called the proAKAP4 or full-length AKAP4.
This monoclonal antibody is suitable for Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry applications.
This monoclonal antibody (clone 5H2) only recognizes the pro-form/precursor of AKAP4 (110 kDa / 854 AA), and the prodomain of 21 kDa (as a results of proAKAP4 conversion into AKAP4) and will not react with the AKAP4 (82 kDa / 665 AA).
This specific monoclonal antibody reacts also with the proAKAP4 proteins from Goat, Camel, Cat, Dog, Pig, Rabbit semens.
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
More references on proAKAP4 antibodies and application protocols:
Carracedo S, Briand-Amirat L, Dordas-Perpinyà M, Ramos Escuredo Y, Delcombel R, Sergeant N and Delehedde M (2022) ProAKAP4 protein marker: towards a functional approach to male fertility. Animal Reproduction Science. Vol.247(107074):1-20. https://doi.org/10.1016/j.anireprosci.2022.107074
Carracedo S, Loyens A, Eddarkaoui S, Serteyn D, Malo C, Skidmore L, Briand-Amirat L, Barbotin AL, Maurage CA, Delehedde M and Sergeant N (2020) The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals. Animal Reproduction Science. Vol. 220 :106448 - P88 https://www.sciencedirect.com/science/article/abs/pii/S0378432020303201
Delehedde M, Carracedo S, Duchene B, Demouveaux B, Remy G, Kervoaze G, Aubry S, Gevaert MH, Maurage CA, Jonckheere N, Van Seuningen I, Philippe Gosset G, Desseyn JL, Sergeant N and Pichavant M (2020) Cigarette smoking affects the sperm-specific proAKAP4 concentrations and impairs both spermatogenesis and sperm quality. Andrology. Vol. 8 (Suppl. 1):45-46.
Delehedde M, Carracedo S, Selleslagh M, Eddarkaoui S, Amirat-Briand L and Sergeant N (2019) ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. Vol. 2(1):13-19.
Delehedde M, Demouveaux B, Remy G, Selleslagh M, Dewulf Q, Desseyn JL, Moreau M, Gosset P, Muriel Pichavant M and Sergeant N (2019a) ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. Vol. 7(1):86.
Langlade C, Buff S, Dias C, Commin L (2020) Assessment of Optimized Frozen/Thawed Semen Samples in Canines with the New A-Kinase Anchor Protein 4 Precursor Biomarker. Biopreserv Biobank. 18(5):409-414.
Sergeant N, Blommaert D, Carracedo S, Franck T, Lejeune JP, Gevaert MH, Aubry S, Maurage CA, Serteyn D and Delehedde M (2020) Assessment of the proAKAP4 concentrations as a pertinent sperm parameter to select extenders and preservatives of stallion semen. Animal Reproduction Science. Vol. 220 :106449. P89. https://www.sciencedirect.com/science/article/abs/pii/S0378432020303213#!
Sergeant N, Carracedo S, Blommaert D, Aubry S, Jouy N, Lejeune JP, Franck T, Maurage CA, Serteyn D, Buée L and Delehedde M (2020) Proteolysis of proAKAP4 in semen as a regulatory sensor of sperm quality and functionality. Andrology. Vol. 8 (Suppl. 1):44-45.