Anti-AKAP4 (clone 7E10) - 100 µg

The anti-AKAP4 mouse monoclonal antibody clone 7E10 recognizes the carboxy-terminal region of A-kinase anchor  protein 4 (AKAP4). This antibody is suitable for Western Blot, for Immunohistochemistry, for ELISA, for Electron Microscopy, for Flow cytometry and Immunofluorescence.

This monoclonal antibody was produced from a mouse hybridoma resulting from a mouse immunized with a peptide covering the C-terminus of human AKAP4 protein sequence (Uniprot ref. Q5JQC9) which is 90% homologous between mammals.

This antibody is determined by its ability to recognise the C-terminus human AKAP4 protein. The monoclonal antibody (clone 7E10) recognizes both the pro-form of AKAP4 (110 kDa / 854 AA) and the AKAP4 (82 kDa / 665 AA) but does not react with the prodomain (21 kDa). 

Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.

Examples of uses :

Scovell JM, Bournat JC, Szafran AT, Solis M, Moore J, Rivera A, Chen CH, Zhang J, Wilken N, Seth A, Jorgez CJ (2021) PRSS50 is a testis protease responsible for proper sperm tail formation and function. Development Vol.148 (8): dev197558.

Carracedo S, Briand-Amirat L, Dordas-Perpinyà M, Ramos Escuredo Y, Delcombel R, Sergeant N and Delehedde M (2022) ProAKAP4 protein marker: towards a functional approach to male fertility. Animal Reproduction Science. Vol.247(107074):1-20.https://doi.org/10.1016/j.anireprosci.2022.107074

Complete list of references using our products upon request at : contact@4biodx.com 

Background on AKAP4 protein

AKAP 4 (A-Kinase Anchor Protein 4) protein belongs to the family of A-kinase anchor proteins (AKAPs) all sharing a common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the PKA holoenzyme to discrete locations within the cell.

The human AKAP4 gene is located on chromosome X and encodes a 854 amino acid polypeptide localized in the fibrous sheath of the sperm flagellum, AKAP4 plays a major role in sperm motility. AKAP4 is also named AKAP-4, AKAP82 (A-Kinase Anchor Protein 82 KDa), PRKA4 (Protein Kinase A Anchoring Protein 4), HI, CT99 (Cancer/Testis Antigen 99), FSC1 (Fibrous sheath component 1) or P82.

Mouse monoclonal antibody clone 7E10 (reference 4BDX-1602) recognizes the carboxy-terminal region of A-kinase anchor protein 4 (AKAP4) and is suitable for Western Blot, for Immunohistochemistry and Immunofluorescence.

According the litterature, sperm from high fertility bulls have higher expression of AKAP4 than low fertility bulls (Peddinti et al. 2008). In humans, a decrease in AKAP4 was observed in nonfertile patients with normal sperm parameters (Frapsauce et al. 2014).

Check out the 4BioDx antibody with the reference 4BDX-1701 (clone 6F12) that recognizes the N-terminal region of A-kinase anchor protein 4 (AKAP4) and is suitable for Western Blot, Immunohistochemistry, ELISA, Electron Microscopy and Immunofluorescence.

More references on AKAP4 antibodies and application protocols: 

Carracedo S, Briand-Amirat L, Dordas-Perpinyà M, Ramos Escuredo Y, Delcombel R, Sergeant N and Delehedde M (2022) ProAKAP4 protein marker: towards a functional approach to male fertility. Animal Reproduction Science. Vol.247(107074):1-20.https://doi.org/10.1016/j.anireprosci.2022.107074

Scovell JM, Bournat JC, Szafran AT, Solis M, Moore J, Rivera A, Chen CH, Zhang J, Wilken N, Seth A, Jorgez CJ (2021) PRSS50 is a testis protease responsible for proper sperm tail formation and function. Development Vol.148 (8): dev197558.

Griffin R, Swegen A, Baker M, John Aitken R, A Skerrett-Byrne D, Silva Rodriguez A, E Martín-Cano F, Nixon B, J Peña F, Delehedde M, Sergeant N and Gibb Z (2020) Mass spectrometry reveals distinct proteomic profiles in high- and low-quality stallion spermatozoa. Reproduction. 160(5):695-707.

Carracedo S, Loyens A, Eddarkaoui S, Serteyn D, Malo C, Skidmore L, Briand-Amirat L, Barbotin AL, Maurage CA, Delehedde M and Sergeant N (2020) The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals. Animal Reproduction Science. Vol. 220 :106448 - P88 https://www.sciencedirect.com/science/article/abs/pii/S0378432020303201

Delehedde M, Carracedo S, Duchene B, Demouveaux B, Remy G, Kervoaze G, Aubry S, Gevaert MH, Maurage CA, Jonckheere N, Van Seuningen I, Philippe Gosset G, Desseyn JL, Sergeant N and Pichavant M (2020) Cigarette smoking affects the sperm-specific proAKAP4 concentrations and impairs both spermatogenesis and sperm quality. Andrology. Vol. 8 (Suppl. 1):45-46.

Langlade C, Buff S, Dias C, Commin L (2020) Assessment of Optimized Frozen/Thawed Semen Samples in Canines with the New A-Kinase Anchor Protein 4 Precursor Biomarker. Biopreserv Biobank. 18(5):409-414.

Riesco M, Anel-Lopez L, Neila-Montero M, Palacin-Martinez C, Montes-Garrido R, Alvarez M, de Paz P, Anel L (2020) ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm. Biomolecules. 10(7):1046. doi: 10.3390/biom10071046.

Ruelle I, Sergeant N, Bencharif D, Charreaux F, Thorin C, Michaud S, Dordas-Perpinyà M, Jouy N, Audry S, Maurage CA, Delehedde M and Briand-Amirat L (2020) ProAKAP4 concentrations in semen as a predictive tool of bull fertility: A preliminary study. Reproduction, Fertility and Development. 32(1):145.

Sergeant N, Blommaert D, Carracedo S, Franck T, Lejeune JP, Gevaert MH, Aubry S, Maurage CA, Serteyn D and Delehedde M (2020) Assessment of the proAKAP4 concentrations as a pertinent sperm parameter to select extenders and preservatives of stallion semen. Animal Reproduction Science. Vol. 220 :106449. P89. https://www.sciencedirect.com/science/article/abs/pii/S0378432020303213#!

Sergeant N, Carracedo S, Blommaert D, Aubry S, Jouy N, Lejeune JP, Franck T, Maurage CA, Serteyn D, Buée L and Delehedde M (2020) Proteolysis of proAKAP4 in semen as a regulatory sensor of sperm quality and functionality. Andrology. Vol. 8 (Suppl. 1):44-45.

Blommaert D, Franck T, Lejeune JF, Selleslagh M, Jouy N, Sergeant N, Serteyn D and Delehedde M (2019b) The Horse 4MID® Kit as a promising tool to compare different freezing media to preserve stallion semen. Rev. Bras. Reprod. Anim. Vol 43(2):585.

Blommaert D, Sergeant N, Delehedde M, Jouy N, Mitchell V, Franck T, Donnay I, Lejeune JP and Serteyn D (2019b) Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology. Vol. 131:52-60.

Delehedde M, Carracedo S, Selleslagh M, Eddarkaoui S, Amirat-Briand L and Sergeant N (2019) ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. Vol. 2(1):13-19.

Delehedde M, Demouveaux B, Remy G, Selleslagh M, Dewulf Q, Desseyn JL, Moreau M, Gosset P, Muriel Pichavant M and Sergeant N (2019a) ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. Vol. 7(1):86.